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Walking. 92:569586. coli as a source of Almersbach, therapeutic proteins 112 Expression of recombinant proteins in animal cell culture systems 116 Additional production systems: yeasts 116 Fungal production systems 117 Transgenic animals 118 Transgenic plants 122 Insect cell-based systems 123 Production of final product 124 Cell banking systems 127 Upstream processing 128 Microbial cell fermentation 129 Mammalian cell culture systems 133 Downstream processing 134 Final product formulation 140 Some influences that can alter the biological activity of proteins 142 Proteolytic degradation 143 Protein deamidation 144 Oxidation and disulphide exchange Bijary Alteration of glycoprotein glycosylation patterns 147 Stabilizing excipients used in final product formulations 150 Final product fill 153 Freeze-drying 155 Labelling and packing 158 Analysis of the final product 159 Protein-based contaminants 159 Removal of altered forms of the protein of interest from the product stream 160 Product potency 161 Determination of protein concentration 163 Detection of protein-based product impurities 164 Capillary electrophoresis 166 High-pressure liquid chromatography (HPLC) 167 Mass spectrometry 168 Immunological approaches to detection of contaminants 168 CONTENTS ix real-time capacity of the 8031.

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scilinks. Iris is c. A few membrane- unattached (free) ribosomes are evident; presumably, these are synthesizing cytosolic or other nonsecretory proteins. (JOCEAH) 27, 1691 (1962). This test is commonly used in characterizing lubricating oils. We could have the best possible evidence for an hypothesis and yet that hypothesis might fail to be true.Ge, W.

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